Wondfo Canine Progesterone Guide: When to Breed for Best Results

Canine Progesterone Timing & Breeding Guide
US Units (ng/mL) Intl. Units (nMol/L) Milestone Breeding Guidance
2–5 6.5–15 LH Surge
Progesterone begins to rise
Begin daily testing, prepare for ovulation
6–10 20–30 Ovulation Estimate
Day 0 for timing
Start ovulation-based breeding plans
11–15 35–50 1st Breeding Window Ideal for natural breeding or AI #1
15–25 50–80 2nd Breeding Window Follow-up AI or second natural tie
30–40 95–125 Surgical Implant Range Only use with high-end lab analyzers
Recommended Breeding Timing (Post-Ovulation):
Natural Tie: 2–3 Days
AI with Fresh Semen: 3–4 Days (ensure sperm health)
AI with Frozen Semen: 4–5 Days (verify motility)

Best Practice: Inseminate twice for optimal conception outcomes.
Conversion: 1 ng/mL = 3.18 nMol/L

Planning your next breeding? The Wondfo Progesterone Rapid Quantitative Test is a powerful tool for breeders who demand precision and reliability. At Stokeshire, where excellence in genetics meets thoughtful care, we use quantitative testing to pinpoint the optimal window for breeding—ensuring every litter has the best possible start.

Whether you're using the Wondfo test at home or in a clinical setting, interpreting results correctly is key. Below, you’ll find our breeder-approved chart that translates progesterone levels into clear breeding milestones—compatible with results from Wondfo’s quantitative analyzer.

We also provide timing tips for natural ties, fresh and frozen AI, and surgical implantation—all in one beautifully simplified reference.